Uses of a Lauryl Sulfate Tryptose Broth for the Detection of Coliform Organisms

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Abstract
Through the use of tryptose, a nutrient which allows the growth of larger numbers of the bacteria originally present in the sample and which also caused the "slow lactose fermenters" to produce gas in larger amounts in shorter time than standard lactose broth, and sodium lauryl sulphate, an agent selective for the coliform group, the authors have obtained a presumptive test medium which gives fewer false positives than does the standard lactose broth. Evidence is also presented that the standard methods confirmatory media, brilliant green bile and E. M. B., may sometimes act as suppressing agents and give colon indices lower than the true value.

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