Inducibility of Lambda Phage Development in Escherichia coli Mutants Thermosensitive for DNA Replication

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Abstract
In a thermosensitive dnaZ mutant lysogenic for λ, induction of the prophage development was provoked at the restrictive temperature. In dnaD strain, spontaneous release of λ proceeded even at 43 °C, but distinct induction of the prophage development was not caused by a heat treatment. Similarly, shift up of growth temperature did not lead to induction of λ in lysogens carrying thermosensitive mutation in dnaH function or DNA polymerase I. In dnaH mutant, multiplication of λ-virulent phage proceeded normally at 43 °C.