Ha‐Ras stimulates uptake and phosphorylation of ethanolamine: inhibition by wortmannin
- 9 January 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 357 (3) , 279-282
- https://doi.org/10.1016/0014-5793(94)01371-7
Abstract
Transformation of NIH 3T3 fibroblasts by Ha-Ras resulted in large increases in the phosphorylation of both [14C]ethanolamine (Etn) and [14C]choline (Cho) when these precursors were added to the medium. Wortmannin, an inhibitor of phosphatidylinositol 3-kinase (P13K), preferentially decreased phosphorylation of externally added Etn in the Ha-Ras transformed, but not in the untransformed, fibroblasts. However, wortmannin had no effect on the phosphorylation of Etn formed endogenously by phorbol ester-stimulated hydrolysis of phosphatidylethanolamine. Data suggest that interaction of mutated Ras with PI3K leads to specific stimulation of Etn uptake, followed by nearly quantitative phosphorylation of Etn by a Ras-activated Cho/Etn kinase.Keywords
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