Interaction of Mercuric Ions with the Bacterial Growth Medium and Its Effects on Enzymatic Reduction of Mercury
- 5 September 1993
- journal article
- Published by Wiley in Biotechnology Progress
- Vol. 9 (5) , 526-532
- https://doi.org/10.1021/bp00023a012
Abstract
The interaction of Hg2+ with components of Luria‐Bertani broth (LB), used to grow the mercury‐resistant strain Pseudomonas aeruginosa PU21 (Rip64), was studied using cold vapor atomic absorption spectrometry and liquid scintillation counting. The time course analysis of free Hg2+ and total Hg in LB solutions showed that 30–40% of Hg2+ formed complexes with tryptone and yeast extract within 140 h. Media containing free or complexed Hg2+ were treated with cell‐free mercuric reductase as well as with growing cells to investigate the effect of the Hg2+‐LB interaction on mercury reduction. In both assays, it was found that the complexed Hg2+ could not be reduced by mercuric reductase or P. aeruginosa PU21 (Rip64).Keywords
This publication has 32 references indexed in Scilit:
- Organomercurial lyase and mercuric ion reductase: nature's mercury detoxification catalystsAccounts of Chemical Research, 1990
- Plasmid-Mediated Heavy Metal ResistancesAnnual Review of Microbiology, 1988
- Bacterial resistance to mercury — reductio ad absurdum?Trends in Biochemical Sciences, 1985
- Mercuric reductase from R-plasmid NR1: characterization and mechanistic studyBiochemistry, 1983
- Binding of mercuric and other heavy metal ions by microbial growth mediaMicrobial Ecology, 1975
- The rate of loss of mercury from aqueous solution when stored in various containersAnalytica Chimica Acta, 1973
- Thiol compounds and resistance of Pyrenophora avenae to mercuryTransactions of the British Mycological Society, 1973
- DNA synthesis in nucleotide-permeable Escherichia coli cells: I. Preparation and properties of ether-treated cellsJournal of Molecular Biology, 1971
- R Factors Mediate Resistance to Mercury, Nickel, and CobaltScience, 1967
- The reaction of thiol and disulphide groups with mercuric chloride and methylmercuric iodide. I. Simple thiols and soluble proteinsAustralian Journal of Chemistry, 1960