Immunohistochemical and biochemical studies of substances with taurine-like immunoreactivity in the brain.
- 1 January 1987
- journal article
- research article
- Published by Japan Society of Histochemistry & Cytochemistry in ACTA HISTOCHEMICA ET CYTOCHEMICA
- Vol. 20 (1) , 31-40
- https://doi.org/10.1267/ahc.20.31
Abstract
Taurine-like immunoreactivity (TLI) in the brain was studied by immunohistochemical and biochemical approaches. The immunohistochemical examination, using different fixatives or taurine antisera preabsorbed with crossreactive substances, demonstrated that TLI staining in the striatum differed from that in the cerebellum. The results suggested that TLI staining should be attributed to unknown substances as well as the amino acid taurine. Immunohistochemical results using a new antiserum raised against .gamma.-glutamyl-taurine supported this assumption. Furthermore, we attempted to extract TLI-positive materials from bovine brain by ion-exchange chromatography and high performance liquid chromatography. A novel immunoreactive substance was isolated and it was distinct immunochemically from either taurine, .gamma.-glutamyl-taurine or glycyl-taurine.This publication has 12 references indexed in Scilit:
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