CHROMATOGRAPHY OF YEAST LIPIDS ON SILICIC ACID
Open Access
- 8 July 1962
- journal article
- research article
- Published by Chartered Institute of Brewers and Distillers in Journal of the Institute of Brewing
- Vol. 68 (4) , 318-321
- https://doi.org/10.1002/j.2050-0416.1962.tb01872.x
Abstract
Yeast lipids have been isolated from whole cells by solvent extraction procedures. The components of the lipid extract have been fractionated by gradient elution chromatography on silicic acid columns, using chloroform-methanol mixtures as eluents. Combined chemical, ultraviolet absorption, paper chromatographic and thin-layer chromatographic techniques have been used to characterize the lipid samples. The results indicate that the neutral lipid fractions contained coenzyme Q, in addition to sterol esters, glycerides, free fatty acids and sterol. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol and carbohydrate-containing lipids were present in the phospholipid fractions.Keywords
This publication has 20 references indexed in Scilit:
- 732. Phospholipids. Part VII. The structure of a monophosphoinositideJournal of the Chemical Society, 1961
- Evidence for the invivo incorporation of 2-amino-2-methylpropanol into rat liver phospholipidBiochemical and Biophysical Research Communications, 1960
- Zur Dünnschicht-Chromatographie der Gehirn-Lipoide, ihrer Um- und AbbauprodukteHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1960
- A Concave Concentration Gradient of Methanol in Chloroform Employed in Elution of Lipids from Silicic AcidNature, 1959
- Studies on the electron transport systemBiochimica et Biophysica Acta, 1959
- UbiquinoneNature, 1958
- The nature of cholesterol esters in human blood serumBiochimica et Biophysica Acta, 1956
- Paper Chromatographic Separation of Phospholipids1Journal of the American Chemical Society, 1955
- The measurement of 32P labelling of individual kephalins and lecithin in a small sample of tissueBiochimica et Biophysica Acta, 1954
- The Isolation of Dipalmitoleyl-L-α-glycerylphosphorylcholine from Yeast. A New Route to (Dipalmitoyl)-L-α-lecithin1Journal of the American Chemical Society, 1952