Plantlet Production from Morphogenetically Competent Cell Suspensions of Daylily*

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Abstract
Cell suspensions of the diploid daylily cultivar Autumn Blaze were produced from larger masses of tissue by culture in the basal medium of Murashige and Skoog supplemented with 10 per cent v/v coconut water and 2 mg 1−12,4–D. By drastically lowering the level of 2,4–D, followed by transferral to a modified White's or Schenk and Hildebrandt medium, clusters grow and ultimately give rise to embryonic structures. A final period in a semi-solid medium stimulates shoot and root growth to the point where successful transplanting of plantlets to soil is assured provided safeguards to prevent ‘damping off’ and desiccation are taken. Normal plantlet formation may be arrested in the formation of neomorphs which do not seem per se to be capable of further development but they can give rise to morphologically normal plantlets after they are stimulated to form callus which, in turn, is given an appropriate sequence of stimuli.