Controlling Protein Orientation at Interfaces Using Histidine Tags: An Alternative to Ni/NTA
- 1 February 2005
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 127 (7) , 2018-2019
- https://doi.org/10.1021/ja045084g
Abstract
A strategy for immobilizing histidine-tagged proteins at surfaces has been developed by using a macrocyclic chelator that enhances the stability and specificity of conventional histidine-tag technology and allows application of this technology to long-term studies including direct electrochemistry.Keywords
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