LIGHT, FLUORESCENT, AND ELECTRON-MICROSCOPIC ANALYSIS OF CULTURED BREAST-TUMOR CELLS (T-47D) TREATED WITH 9,10-ANTHRACENEDICARBOXALDEHYDE BIS[(4,5-DIHYDRO-1H-IMIDAZOL-2-YL)HYDRAZONE] DIHYDROCHLORIDE

  • 1 January 1983
    • journal article
    • research article
    • Vol. 43  (12) , 5910-5914
Abstract
The influence of bisantrene on T-47D human breast tumor cells was assessed by colony-forming assay in soft agar and by light microscopy, fluorescence microscopy and EM. Test solutions of bisantrene solubilized in distilled water or dimethyl sulfoxide [DMSO] were added to cultures at final concentrations between 0.01-60 .mu.g/ml. Brightly fluorescent particles appeared in a concentration-dependent fashion after cultures were treated with water-soluble bisantrene at concentrations > 0.1 .mu.g/ml. Similar fluorescent crystals appeared in culture media when concentrations of the DMSO-dissolved drug exceeded 10 .mu.g/ml. Clonogenic survival as defined by soft agar assay indicated significant reproductive impairment in cells treated with concentrations > 1 .mu.g/ml (P < 0.01). Nuclear and cytoplasmic fluorescence was evident in treated cells. Cells that survived 24-h drug treatment displayed round nuclei with watery nucleoplasm when examined under the light microscope. Under EM, nuclei of these cells revealed fragmentation of the nucleolar complex and a highly electron-lucent nucleoplasm. Cytoplasmic responses, which seem to be relatively innocuous, include incorporation of the fluorescent crystals into lysosomes and some mitochondrial abnormalities. Crystalline inclusions engulfed by lysosomes were found in cells obtained from cultures showing bisantrene precipitates.

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