ENZYMATIC ASSAY OF CREATININE IN SERUM - COMPARISON WITH JAFFE METHODS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 17  (11) , 683-687
Abstract
The creatinine concentrations in 337 [human] sera were determined by enzymatic assay and by 6 different modifications of the Jaffe method. The enzymatic assay showed satisfactory precision and gave the same results as the assay after adsorption on Fuller''s earth (Lloyd''s reagent). All other methods using picrate gave falsely high values; the relative deviation from the enzymatic method had a median value between +20 and +50% for creatinine concentrations in the range 22-88 .mu.mol/l, and between +10 and +30% for creatinine concentrations in the range 89-177 .mu.mol/l. The study thus confirms the lack of specificity of all Jaffe methods, with the exception of the assay after adsorption on Fuller''s earth.

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