Denaturation of the tryptic fragments of the calcium(II) adenosine triphosphatase from sarcoplasmic reticulum by guanidinium hydrochloride

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Abstract
Primary and secondary fragments of the Ca2+-ATPase from sarcoplasmic reticulum are resistant to complete denaturation by guanidinium hydrochloride, a property characteristic of many intrinsic membrane proteins. None of the fragments display a single cooperative transition from ordered structure to random coil suggesting each fragment contains several domains of differing resistance to guanidinium hydrochloride denaturation. The native enzyme had at least 3 membrane-embedded domains, with an externally accessible link between each.