Effects of Dehydroepiandrosterone and Cyano-Ketone on Ovarian Weight, Cholesterol Content, and Ultrastructure in PMS-HCG Treated, Immature Rats
- 1 November 1971
- journal article
- other
- Published by The Endocrine Society in Endocrinology
- Vol. 89 (5) , 1223-1235
- https://doi.org/10.1210/endo-89-5-1223
Abstract
Immature rats were given the PMSHCG treatment recommended by Parlow for the preparation of rats for the ovarian ascorbic acid depletion (OAAD) assay of LH. At 30 days of age one group of 5 rats received two 5 mg injections of cyano-ketone (a potent inhibitor of δ5- 3β-hydroxysteroid dehydrogenase) and two other groups were given cyano-ketone (CK) followed by daily injections of 15 or 20 mg of dehydroepiandrosterone (DHA) for seven days. Finally, one group was given the daily DHA treatment (20 mg) but received no CK. At autopsy, ovaries were weighed and either analyzed for total cholesterol content or prepared for ultrastructural study. The CK treatment did not significantly alter either the ovarian weight or cholesterol content. All three groups given DHA showed a remarkable enlargement of the heavily luteinized ovaries and a significant reduction in ovarian cholesterol concentration as well as in total ovarian cholesterol. These changes were accompanied by ultrastructural signs of heightened secretory activity in the luteal cells suggesting that the DHA treatment had either directly or indirectly stimulated the secretion of prolactin from the pituitary gland. (Endocrinology89: 1223, 1971)Keywords
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