Measurement of "Free" Thyroxine in Serum
- 1 March 1971
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 17 (3) , 166-173
- https://doi.org/10.1093/clinchem/17.3.166
Abstract
A dialysis method for determining "free" thyroxine in diluted serum is presented. The effect of a number of variables (pH, incubation time and temperature, buffer composition and concentration, protein concentration, and specimen dilution) was studied, and pertinent data are presented. Within-run precision (95% confidence limits) was ±10.6%; between-run precision was ±14.2%. Specimens from 84 healthy subjects were analyzed, and normal ranges are presented. The method has been used for five years, and has been found to be simple and suited to simultaneous analysis of large numbers of specimens.Keywords
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