HLA-A2.1-Associated Peptides from a Mutant Cell Line: A Second Pathway of Antigen Presentation
- 6 March 1992
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 255 (5049) , 1264-1266
- https://doi.org/10.1126/science.1546329
Abstract
Peptides extracted from HLA-A2.1 class I major histocompatibility complex (MHC) molecules expressed on the antigen processing mutant CEMx721.174.T2 were characterized by electrospray ionization-tandem mass spectrometry. Only seven dominant peptides were found, in contrast to over 200 associated with HLA-A2.1 on normal cells. These peptides were derived from the signal peptide domains of normal cellular proteins, were usually larger than nine residues, and were also associated with HLA-A2.1 in normal cells. These results suggest that proteolysis of signal peptide domains in the endoplasmic reticulum is a second mechanism for processing and presentation of peptides for association with class I molecules.Keywords
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