Immune Response to Gross Virus-Induced Lymphoma. III. Characteristics of the Cellular Immune Response

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Abstract
An in vitro cellular cytotoxicity assay was used to study the development of cellular immunity to a syngeneic Gross virus-induced lymphoma in W/Fu rats. Cellular immunity was first detected in regional lymph node cells, 4 days after immunization. Reactivity developed later in peripheral blood lymphocytes, spleen cells, and peritoneal cells. Distal lymph nodes, thymus, and bone marrow had little or no cytotoxic effects. Each reactive cell population reached a peak of cytotoxic activity 6–14 days after immunization; then cytotoxicity declined rapidly. By tests of various attacker: target cell ratios, quantitative curves of immune activity for each cell population were obtained. The peripheral blood lymphocytes had higher activity and a more shallow slope than the other lymphoid organs. Purification of the lymphoid cells increased cytotoxic effects, but the differences in reactivity among the different lymphoid populations could not be entirely accounted for by the percent lymphocytes in the preparations. The cytotoxic immune cells were resistant to X irradiation, with persistent activity after 4000 R.

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