Purification and properties of adenosine triphosphatase solubilized from beef heart mitochondria by chloroform
- 1 December 1977
- journal article
- research article
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 18 (2-3) , 77-80
- https://doi.org/10.1007/bf00280271
Abstract
Summary Soluble, oligomycin-insensitive ATPase released from beef heart mitochondria by chloroform extraction can be further purified by Sepharose 6B gel filtration. This purification increases enzyme activity 4–5 times (100–130 U/mg). According to specific activity, high purity and ability to reconstitute oligomycin-sensitive complex, isolated ATPase is quite comparable with enzyme preparations isolated by other methods.Keywords
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