D-MANNOSE AS A CONSTITUENT OF THE DNA OF A MUTANT STRAIN OF BACTERIOPHAGE SP8

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Abstract
Bacteriophage SP8 and its host range, temperature-sensitive mutant SP8*TS, yield two strands of different density when denat-urated. In both cases, the C/P ratio of the "light" strand is considerably higher than that of the "heavy" strand which may help explain the observed different bouyant densities. Analyses showed that the DNA of both phages are glycosylated. Large quantities of hexose (>1 mole/P) would have to be on the "light" strand to account for the different C/P ratios found. It was found that SP8 contains D-glucose (0.98 moles/P) and the mutant, SP8*Ts, contains D-mannose (0.81 moles/P).