Negative Interference by Residual Proteins in the Supernatant Fluid Used in Radioisotopic Assay of Serum Vitamin B12

1 September 1972

journal article
research article
Published by Oxford University Press (OUP) in Clinical Chemistry

Vol. 18 (9) , 895-900
https://doi.org/10.1093/clinchem/18.9.895

Abstract

Separation of vitamin B₁₂, from serum by heat denaturation results in an individually variable amount of residual protein in the supernatant fluid. I show that during radioisotopic assay of serum vitamin B₁₂, such protein may seriously interfere with the results, and that the interference may be abolished by changing the experimental conditions.

Keywords

INTRINSIC FACTOR BINDING CAPACITY
SEPARATION OF VITAMIN B12 BY HEAT DENATURATION OF SERUM
BOUND AND UNBOUND B12
PROTEIN-COATED CHARCOAL REAGENT
BINDING KINETICS

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