Effect of Feeding “Carbohydrate-free” Diets on the Chick's Requirement for Methionine

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Abstract
Experiments were conducted to study the effect of fat on the chick's requirement for methionine when fat was substituted isocalorically for carbohydrate in a semipurified diet in which protein was supplied by isolated soybean protein. Using either growth or energetic efficiency as the criterion, the chick's requirement for methionine was shown to remain unchanged when hydrogenated fat was substituted isocalorically for glucose, irrespective of the vitamin B-12 status of the chick. Thus, the increased requirement for vitamin B-12 which has been previously observed when fat was substituted isocalorically for glucose cannot be attributed to increased need for methionine, and may reflect a role for vitamin B-12 in fat metabolism.