INVIVO QUANTITATION AND KINETICS OF RABBIT NEUTROPHIL LEUKOCYTE ACCUMULATION IN THE SKIN IN RESPONSE TO CHEMOTACTIC AGENTS AND ESCHERICHIA-COLI

  • 1 January 1980
    • journal article
    • research article
    • Vol. 42  (3) , 310-317
Abstract
The in vivo quantitation of neutrophil accumulation at inflammatory sites in rabbits by employing 51Cr-labeled autologous rabbit blood neutrophils is described. These labeled neutrophils circulated with a half-life of 3.2-3.8 h. They localized with great specificity at skin sites injected intradermally [i.d.] with zymosan-activated plasma, synthetic chemotactic peptides (e.g., N-formyl methionyl leucyl phenylalanine), E. coli-derived chemotactic factors or whole E. coli. Contrary to reports utilizing in vitro assays, under in vivo conditions the synthetic chemotactic peptides caused significantly less neutrophil accumulation than zymosan-activated plasma or E. coli chemotactic factors. No inhibition of neutrophil accumulation by high concentrations of N-formyl methionyl leucyl phenylalanine was observed. Kinetic studies of the accumulation of labeled leukocytes in the skin in response to i.d. injection of formalin-killed E. coli were performed. Nearly all leukocytes accumulated during the first 4 h after E. coli injection with a peak rate of accumulation between 2 and 3 h. Essentially no additional localization of leukocytes occurred at lesions which were 6, 8 or 24 h old. 51Cr-labeled rabbit blood neutrophils can be utilized to quantitate the degree of neutrophil accumulation in inflammatory reactions and to study the hour by hour kinetics of this accumulation.

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