Ver nderungen des Chlorophyllgehaltes und der Chloroplastenfeinstruktur in den Hochbl ttern von Davidia involucrata Baill.
- 1 January 1967
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 76 (4) , 335-347
- https://doi.org/10.1007/bf00387539
Abstract
This paper deals with the destruction of plastid pigments and of chloroplast during the bleaching of the bracts of Davidia involucrata Baill. Changes in pigment content have been analzyed both spectrophotometrically and by thin layer chromatography, whereas changes in the fine structure of leaf pieces have been examined by electron microscopy. After fixation in glutaraldehyde and osmium tetroxide, the leaf pieces were embedded in Epon. Thin sections were stained with lead citrate and examined under a Siemens Elmiskop I. In bracts and in normal leaves of Davidia 2 water soluble pigments were present which have been identified as flavone glycosides. The qualitative composition of the pigments in leaves and in different stages of the bracts was identical. During the 18 days of observation the surface of the bracts increased about 10 fold. The chlorophylls, on the contrary, were completely decomposed. No characteristic changes in the ratio a/b could be detected. With the beginning decomposition of chlorophyll, an alteration of the fine structure of the chloroplast was noted. The piles of thylakoids showed a swelling which began on the outside. At the same time the number of thylakoids was reduced. "Vacuoles" appeared in the stroma, and the osmiophilic globules increased in number. Under natural conditions, the grana-thylakoids in the chloroplasts of bracts from Davidia were disintegrated before the stroma-thylakoids These findings are contrary to those of other authors.This publication has 32 references indexed in Scilit:
- NUCLEIC ACIDS OF CHLOROPLASTS AND MITOCHONDRIA IN SWISS CHARDThe Journal of cell biology, 1965
- Thin-Layer Chromatography of Plant Pigments on Mannitol or SucroseScience, 1965
- THE FINE STRUCTURE OF CHLOROPLAST STROMA FOLLOWING ALDEHYDE OSMIUM-TETROXIDE FIXATIONThe Journal of cell biology, 1965
- Ribosomes in spinach chloroplastsExperimental Cell Research, 1963
- A specific species of ribosomes associated with the chloroplasts of Euglena gracilisBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1962
- Isolation of ribosomes from spinach chloroplastsExperimental Cell Research, 1962
- Licht- und elektronenmikroskopische Untersuchungen an DesmidiaceenPlanta, 1961
- Die Feinstruktur der Plastiden vonTradescantia albiflora (Kth.) bei EisenmangelchloroseProtoplasma, 1961
- The ultrastructure of chloroplastsExperimental Cell Research, 1955
- Zur Struktur erblich verschiedener Chloroplasten vonOenotheraProtoplasma, 1955