The effect of paromomycin and [psi] on the suppression of mitochondrial mutations in Saccharomyces cerevisiae

Abstract
Paromomycin has been found to suppress certain nonsense mutations located in several mitochondrial genes in yeast. In the mosaic genes, paromomycin preferentially suppresses those mutations located in the introns. There is a strong correlation between this phenotypic suppression by paromomycin and the genetic suppression due to various informational mitoribosomal suppressors. No effect of the cytoplasmic element [psi] on mitoribosomal protein synthesis was observed. This work provides strong evidence for the translation of mRNA maturase proteins from open reading frames of the mitochondrial introns.