ENHANCEMENT OF HUMAN POLYMORPHONUCLEAR LEUKOCYTE ADHERENCE BY THE PHOSPHOLIPID MEDIATOR 1-O-HEXADECYL-2-ACETYL-SN-GLYCERO-3-PHOSPHORYLCHOLINE (AGEPC)

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  • 1 January 1983
    • journal article
    • research article
    • Vol. 113  (1) , 85-89
Abstract
The phospholipid mediator 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphorylcholine (AGEPC) increases human polymorphonuclear leukocyte (PMN) adherence to Sephadex G-25 in a manner directly proportional to the concentration of AGEPC from 2-650 nM. The maximal enhancement of PMN adherence of 85 .+-. 23% (mean .+-. SD) evoked by 650 nM APEGC was indistinguishable in magnitude from that elicited by an optimal concentration of the chemotactic peptide N-f-Met-Leu-Phe. The results of analyses of the relative PMN adherence-enhancing activities of analogs of AGEPC suggest distinct molecular requirements for the enhancement of adherence. PMN adherence increases within 1 min after exposure of the leukocytes to AGEPC, and the augmented adherence persists for over 30 min in spite of extensive washing of the leukocytes. Indomethacin [an antiinflammatory drug] partially blocked enhancement of adherence by AGEPC, suggesting that thromboxane A2 mediates in part the enhancement of adherence by AGEPC.

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