Pectinesterase extraction from sharabi apples

Abstract
Pectinesterase (PE) was extracted from apples (cv. Sharabi) and assayed. pH and NaQ concentration influenced the extraction process of PE from the apple fruit. The highest PE extraction value at pH 7.5 and with 1.5 M‐NaCl solution was 4.2 μ equiv.‐COOH/min/g fresh fruit. Lower extraction values were found both with 2‐5 M‐NaCl and distilled water. Treatment of the tissue at pH 3.25 and pH 6.5 resulted in similar extraction values.