Stabilization of 20.BETA.-hydroxysteroid dehydrogenase by glycerol.
- 1 January 1980
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 28 (1) , 314-318
- https://doi.org/10.1248/cpb.28.314
Abstract
20β-Hydroxysteroid dehydrogenase from Streptomyces hydrogenans is partially inactivated in sodium phosphate buffer within a short time. Inactivation of the enzyme was accelerated at acidic pH, and the enzyme was also inactivated by incubation with rose bengal. The inactivation due to acidic pH and rose bengal was prevented by glycerol ; 20% glycerol completely restored the activity lost at pH 5.5. The mechanism of stabilization of the enzyme by glycerol is not yet clear.Keywords
This publication has 4 references indexed in Scilit:
- 20beta-Hydroxysteroid oxidoreductase. Kinetics and binding of corticosteroids and corticosteroid-21-aldehydes.Journal of Biological Chemistry, 1977
- Reversible Cold Inactivation of a 17β-Hydroxysteroid Dehydrogenase of Human Placenta: Protective Effect of Glycerol*Biochemistry, 1966
- The influence of sulfhydryl groups on the stability of carbamylphosphate synthetaseBiochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects, 1964
- 20β-Hydroxy-steroid-dehydrogenase, ein neues kristallines enzymBiochimica et Biophysica Acta, 1959