Standard Solutes for Classification of Solvent Systems in Countercurrent Chromatography

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Abstract
A series of p-dinitrophenylamino alcohols have been synthesized for use as standard solutes for evaluating the polarity of solvent systems used in countercurrent chromatography. The rank order polarity of the DNP-amino alcohols as well as p-nitrophenyl glucosides were determined by measuring their partition coefficients in 1-pentanol/heptane/water systems containing from 2% pentanol in heptane to 100% pentanol. The standard solutes were used to establish the rank order polarity of eleven chloroform/methanol/water systems which are widely used in countercurrent chromatography.