COMPARISON OF WHOLE-BLOOD AND PURIFIED CANINE LYMPHOCYTES IN A LYMPHOCYTE-STIMULATION MICROASSAY

  • 1 January 1978
    • journal article
    • research article
    • Vol. 39  (4) , 687-690
Abstract
The optimum mitogen concentration and time required for using whole blood from dogs in a microassay were determined. This test then was compared with a standard lymphocyte-stimulation microtest, using gradient-isolated lymphocytes, 2 different mitogens (phytohemagglutinin and concanavalin [Con] A) and 2 different culture media. Statistical analysis of the data from 10 dogs showed that whole blood was significantly more reactive than were gradient-isolated lymphocytes (P < 0.05). Waymouth''s medium was significantly better than RPMI 1640 (P < 0.001), and Con A was significantly more mitogenic than phytohemagglutinin (P < 0.001). The interaction between lymphocyte source and mitogens was the only one of the various interactions that was significant at P < 0.05.

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