A Study of the Acid Precipitation of Diphtheria Toxin
Open Access
- 1 June 1931
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 20 (6) , 417-431
- https://doi.org/10.4049/jimmunol.20.6.417
Abstract
Summary: A study has been made of yields of diphtheria toxin obtained by acid precipitation of the crude toxin. A comparison has been made between the yields obtained when the acid precipitate is centrifuged immediately after acidification and when twenty-four hours storage in the cold intervenes between the acidification and the centrifugation. Results are obtained which indicate that a satisfactory method for large scale purification of diphtheria toxin would consist in the addition of acid to the point of maximum precipitation followed by immediate centrifugation, washing and solution of the precipitate at the desired concentration.Keywords
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