Use of Tetramethylbenzidine in Plasma Hemoglobin Assay

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Abstract
Plasma hemoglobin can be assayed by substituting 3,3',5,5'-tetramethylbenzidine for benzidine (a carcinogen) as the chromogenic reducing substance. Sensitivity, precision, and accuracy compare well with methods in which benzidine is used. Plasma inhibition can be ac¬commodated by preincubation with hydrogen peroxide.