Technical Aspects of Immunohistochemistry
Top Cited Papers
Open Access
- 1 July 2005
- journal article
- review article
- Published by SAGE Publications in Veterinary Pathology
- Vol. 42 (4) , 405-426
- https://doi.org/10.1354/vp.42-4-405
Abstract
Immunohistochemistry is an integral technique in many veterinary laboratories for diagnostic and research purposes. In the last decade, the ability to detect antigens (Ags) in tissue sections has improved dramatically, mainly by countering the deleterious effects of formaldehyde with antigen retrieval (AR) and increasing sensitivity of the detection systems. In this review, I address these topics and provide an overview of technical aspects of immunohistochemistry, including those related to antibodies (Abs) and Ags, fixation, AR, detection methods, background, and troubleshooting. Microarray technology and the use of rabbit monoclonal Abs in immunohistochemistry are also discussed.Keywords
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