A comparative ultrastructural and histochemical study of the metacercarial cyst walls of four species ofParagonimus (Troglotrematidae: Trematoda)

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Abstract
The metacercarial cyst walls ofParagonimus westermani, P. miyazakii, P. ohirai, andP. iloktsuenensis were examined using ultrastructural and histochemical techniques. The cyst walls ofP. westermani, P. miyazakii, andP. ohirai were found to have five distinct layers, whereas the wall ofP. iloktsuenensis had only two. The so-called outer cyst wall recognized on light microscopy by Miyazaki (1961) was composed of layers I–III and the so-called inner cyst wall comprised layers IV and V. The outermost layer (I) consisted of collagen fibers and probably originated from the host. Layer III detached easily from layer IV on removal of the cysts from the host tissues. Layer IV was composed of proteoglycans, and layer V consisted of protein alone. A regular hexagonal pattern in layer V appeared to be characteristic ofP. westermani. Although the basic morphological patterns observed in the layers constituting the cyst walls were similar in all species examined, the thickness of each layer was apparently different. This characteristic seems to be a suitable criterion for the identification of metacercarial cysts ofParagonimus species.

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