Liquid-chromatographic profiling of endogenous fluorescent substances in sera and urine of uremic and normal subjects.
Open Access
- 1 April 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 29 (4) , 675-677
- https://doi.org/10.1093/clinchem/29.4.675
Abstract
Endogenous fluorescent substances increase in serum during uremia. We have used "high-performance" liquid chromatography to profile these fluorescent substances in both uremic and normal body fluids. The fluorescence excitation and emission maxima we used were 322 and 415 nm, respectively. Of the numerous fluorescent substances found in uremic body fluids and in normal urine, some were also detectable in normal serum, but at relatively weak fluorescence intensities.Keywords
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