Expression of natural killer (NK) cell‐specific alloantigens on a mouse NK‐like cell line

Publisher Website
Google Scholar
Abstract
As part of the strategy for screening for natural kilter (NK) cell-specific monoclonal antibodies (MoAb) we have raised a number of murine NK-like cell lines in media containing interleukin-2 (IL-2). The detection of specific NK cell alloantigens on a C57BL/6 cell line in long-term culture in IL-2 is the subject of this paper. The C57BL/6 cell line has the morphology of large granular lymphocytes (LGL) and exhibits strong cytolytic activity against the archetype NK cell target, YAC-1, Absorption of three anti-NK antiserum. NZB anti-BALB/c (anti-NK-2-1), BALB/c anti-DBA/2 (anti-NK-31) and CE anti-CBA (anti-NK-41), with the C57BL/6 cell line removed the anti-NK activity from these antisera. Flow cytometric studies of the C57BL/6 cell line demonstrated significant binding of the anti-NK-11 MoAb produced by hybridoma PK136. Our results suggest that the C57BL/6 NK-like cell line exhibits some of the properties of naive NK cells and expresses all the known NK cell-specific alloantigens, NK-11, NK-21. NK-31 and NK-41 and therefore is potentially useful in selecting NK specific hybridomas and in studying the biology of NK cells.