One-step real-time duplex reverse transcription PCRs simultaneously quantify analyte and housekeeping gene mRNAs
Open Access
- 1 March 2004
- journal article
- research article
- Published by Taylor & Francis in BioTechniques
- Vol. 36 (3) , 508-519
- https://doi.org/10.2144/04363rn06
Abstract
We developed a one-step real-time duplex reverse transcription PCR (RT-PCR) method using the LightCycler® platform. This method allows simultaneous reverse transcription and real-time PCR amplifica... Duplex PCR for Diagnostics Of methods used to quantify mRNA, real-time RT-PCR is considered the most sensitive and accurate and has the potential for broad application in diagnosis of functional ge...Keywords
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