Synthesis of oligodeoxynucleotides containing 5-aminouracil and its N-acetyl derivative
- 1 January 1997
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Perkin Transactions 1
- No. 14,p. 2051-2058
- https://doi.org/10.1039/a701371j
Abstract
The preparation of oligonucleotides containing 5-amino-2′-deoxyuridine is described. Three different protective groups for the amino function of 5-aminouracil including trifluoroacetyl, dimethylformamidine and 2-(4-nitrophenyl)ethoxycarbonyl are analysed in order to reduce the acetylation of this base observed during the assembly of oligonucleotides containing this base analogue. The side-reaction is avoided by using the base-labile 2-(4-nitrophenyl)ethoxy as protecting group and 2-(4-nitrophenyl)ethyl chloroformate during the capping step.Keywords
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