PROSTAGLANDIN RELEASE FROM CAT AND DOG SPLEEN

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Abstract
1 The output of prostaglandins from the spleens of cats and dogs was studied. Comparison is made between the results found in the two species. 2 The release of prostaglandins was investigated in isolated saline-perfused spleens and in incubates of spleen slices. Release in response to nerve stimulation, and exposure to adrenaline or noradrenaline was compared with resting release. 3 A resting release of prostaglandins was found in the dog but not in the cat spleen. 4 Whereas stimulated dog spleens released microgram quantities of prostaglandins E2 and F2α, prostaglandin output from the cat spleen under similar conditions was undetectable or barely detectable. 5 The identity of the prostaglandins released from the dog spleen (prostaglandins E2 and F2α) was confirmed by mass spectrometry. 6 The species difference in prostaglandin output from the spleen is discussed in relation to the hypothesis that endogenous prostaglandins modify the responses of this organ to nervous stimuli.