Transcriptome analysis for Caenorhabditis elegansbased on novel expressed sequence tags
Open Access
- 8 July 2008
- journal article
- Published by Springer Nature in BMC Biology
- Vol. 6 (1) , 30
- https://doi.org/10.1186/1741-7007-6-30
Abstract
We have applied a high-throughput pyrosequencing technology for transcriptome profiling of Caenorhabditis elegans in its first larval stage. Using this approach, we have generated a large amount of data for expressed sequence tags, which provides an opportunity for the discovery of putative novel transcripts and alternative splice variants that could be developmentally specific to the first larval stage. This work also demonstrates the successful and efficient application of a next generation sequencing methodology.Keywords
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