Determination of the genome size ofEhrlichiaspp., using pulsed field gel electrophoresis
Open Access
- 1 July 1999
- journal article
- research article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 176 (1) , 73-78
- https://doi.org/10.1111/j.1574-6968.1999.tb13644.x
Abstract
Ehrlichiae are obligatory intracellular, Gram-negative bacteria which belong to the alpha subclass of the phylum Proteobacteria and are responsible for infectious diseases of humans. Little is known about genetics and genomic organization of Ehrlichia spp. The genome sizes of four representatives of the genus Ehrlichia were determined for the first time by pulsed field gel electrophoresis. The sizes for E. sennetsu, E. risticii, E. chaffeensis (strain Arkansas and strain 91HE17), and the HGE agent were 878.5 kb, 880.3 kb, 1225.8 kb, 1262.3 kb and 1494 kb respectively.Keywords
This publication has 11 references indexed in Scilit:
- Purification of Cowdria ruminantium Organisms for Use in Genome Analysis by Pulsed-field Gel Electrophoresis aAnnals of the New York Academy of Sciences, 1998
- Emergence of the Ehrlichioses as Human Health ProblemsEmerging Infectious Diseases, 1996
- Characterization of the SF Agent, an Ehrlichia sp. Isolated from the Fluke Stellantchasmus falcatus, by 16S rRNA Base Sequence, Serological, and Morphological AnalysesInternational Journal of Systematic and Evolutionary Microbiology, 1996
- "Analysis of the Anaplasma mavginale genome by pulsed-field electrophoresis"Journal of General Microbiology, 1993
- Genotypic identification and phylogenetic analysis of the spotted fever group rickettsiae by pulsed-field gel electrophoresisJournal of Bacteriology, 1993
- Determination of genome size and restriction pattern polymorphism ofRickettsia prowazekiiandRickettsia typhiby pulsed field gel electrophoresisFEMS Microbiology Letters, 1993
- The tribe Ehrlichieae and ehrlichial diseasesClinical Microbiology Reviews, 1991
- 16S ribosomal DNA amplification for phylogenetic studyJournal of Bacteriology, 1991
- Pulsed-field gel electrophoresis determination of the genome size of obligate intracellular bacteria belonging to the genera Chlamydia, Rickettsiella, and PorochlamydiaJournal of Bacteriology, 1989
- Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresisCell, 1984