Cloning, sequencing and analysis of the yeastS. uvarum ERG10 gene encoding acetoacetyl CoA thiolase
- 1 June 1988
- journal article
- research article
- Published by Springer Nature in Current Genetics
- Vol. 13 (6) , 471-478
- https://doi.org/10.1007/bf02427752
Abstract
TheERG10 gene specific toS. uvarum, a brewing yeast, has been cloned by complementation of anS. cerevisiae erg10 mutant.S. uvarum contains two differentERG10 genes. One of these is similar to theS. cerevisiae ERG10 gene; they are structurally different, but functionally homologous. The clonedERG10 gene has been located on chromosome XVI, and we have shown that it is allelic to the previously isolatedtsm0115 mutants. Northern blot and sequence analysis indicate that theERG10 gene is highly expressed, and biochemical and genetic evidence show that it encodes the cytoplasmic acetoacetyl CoA thiolase.Keywords
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