One-step selection of Vaccinia virus-binding DNA aptamers by MonoLEX
Open Access
- 1 January 2007
- journal article
- Published by Springer Nature in BMC Biotechnology
- Vol. 7 (1) , 48
- https://doi.org/10.1186/1472-6750-7-48
Abstract
As a new class of therapeutic and diagnostic reagents, more than fifteen years ago RNA and DNA aptamers were identified as binding molecules to numerous small compounds, proteins and rarely even to complete pathogen particles. Most aptamers were isolated from complex libraries of synthetic nucleic acids by a process termed SELEX based on several selection and amplification steps. Here we report the application of a new one-step selection method (MonoLEX) to acquire high-affinity DNA aptamers binding Vaccinia virus used as a model organism for complex target structures.Keywords
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