Biosynthesis of nucleic acids in Bacillus megaterium. 1. The isolation of a nuclear material

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Abstract
Methods are described for fractionating protoplasts of B. megaterium into a nuclear material, a cytoplasmic fraction and a membrane fraction. About 90-95% of the cellular deoxyribonucleic-acid (DNA) is isolated with the nuclear fraction, the ribonucleic-acid (RNA) DNA: protein proportions of which are 1: 7: 10. Nearly all of the cellular ribosomes and over 90% of the total RNA are present in the cytoplasmic fraction. The membrane fraction is made up of clean cytoplasmic membranes with very little RNA or other material attached. Basic protein cannot be extracted from the nuclear fraction.