Subcellular distribution of enzymes involved in the biosynthesis of cyanelle murein in the protist Cyanophora paradoxa

Abstract
Cyanelle containing organisms, notably Cyanophora paradoxa, best studied among them, are unique with respect to the occurrence of peptidoglycan (murein) within an eukaryotic cell. Enzyme activities involved in the biosynthesis of UDP‐N‐acetyl‐muramylpentapeptide could be localized within the cyanelle compartment. Some of the enzymes performing later steps of murcin biosynthesis were detected in the postcyanelle supernatant rather than in the cyanelle lysate. This is taken to reflect a ‘periplasmic’ location of these enzymes that are partially liberated upon rupture of the cyanelle outer membrane.