Direct characterization of bovine microsatellites from cosmids: polymorphism and synteny mapping
- 1 August 1994
- journal article
- Published by Wiley in Animal Genetics
- Vol. 25 (4) , 209-214
- https://doi.org/10.1111/j.1365-2052.1994.tb00195.x
Abstract
A (TG)8 oligonucleotide probe was used to screen 186 cosmids from a commercial bovine cosmid library. Of the 56 positive discovered, 7 were sequenced in the region of the microsatellite and analysed for polymorphism. These microsatellites, IDVGA‐2, ‐3, ‐7, ‐8, ‐9, ‐10, ‐11 showed the following number of alleles and polymorphism information content (PIC) values (7/0.616, 8/0.693, 6/0.641, 5/0.643, 2/0.239, 10/0.844, 6/0.720). The microsatellites were also assigned to synteny groups as follows: IDVGA‐2/U17, IDVGA‐3/U16, IDVGA‐7/U7, IDVGA‐8/U29, IDVGA‐9/U3, IDVGA‐10/U19, IDVGA‐11/A (probably U18).Keywords
This publication has 14 references indexed in Scilit:
- Further characterization of a somatic cell hybrid panel: ten new assignments to the bovine genomeAnimal Genetics, 1994
- Fifteen new synteny assignments of microsatellites to the bovine genomeAnimal Genetics, 1994
- Morphological development and sex of bovine in vitro‐fertilized embryosMolecular Reproduction and Development, 1992
- Survey of human and rat microsatellitesGenomics, 1992
- Microsatellites for linkage analysis of genetic traitsTrends in Genetics, 1992
- The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related speciesGenomics, 1991
- Evolution and distribution of (GT)n repetitive sequences in mammalian genomesGenomics, 1991
- The bovine genome contains polymorphic microsatellitesGenomics, 1990
- Statistical decision rules concerning synteny or independence between markersCytogenetic and Genome Research, 1986
- Bovine 1.709 satelliteJournal of Molecular Biology, 1984