RAPID SIMULTANEOUS RADIOMMUNOASSAY FOR THE MEASUREMENT OF TRIIODOTHYRONINE AND THYROXINE IN UNEXTRACTED HUMAN SERUM
- 1 February 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 81 (2) , 487-494
- https://doi.org/10.1530/acta.0.0810487
Abstract
A rapid simultaneous radioimmunoassay for the measurement of triiodothyronine T3 and thyroxine (T4) in unextracted human serum is described. The method requires 25 .mu.l serum. Approximately 150 human sera can be tested for T3 and T4 levels in 1 day by 1 technical assistant. The T3 levels in peripheral venous blood in euthyroid subjects and in patients with various thyroid diseases are as follows: euthyroid (50) 115 .+-. 22 (mean .+-. SD) ng/100 ml, hypothyroid (20) 33 .+-. 35 (mean .+-. SD) ng/100 ml and hyperthyroid (20) 411 .+-. 142 (mean .+-. SD) ng/100 ml. The corresponding levels for T4 are: 6.9 .+-. 1.3, 2.0 .+-. 1.4 and 21.4 .+-. 5.2 .mu.g/100 ml. The levels of T3 in the thyroid venous blood are higher (P < 0.05) than in the peripheral venous blood. The levels of T4 in the thyroid venous blood was not significantly higher than in peripheral venous blood.This publication has 1 reference indexed in Scilit:
- The measurement of digitoxin in human serum by radioimmunoassayJournal of Clinical Investigation, 1968