Immunoperoxidase staining of Legionella pneumophila

Publisher Website
Google Scholar
Abstract
Immunoperoxidase staining was applied to sections of pneumonic lung from a previously published case of Legionnaires'' disease. Specific staining of L. pneumophila was accomplished with sub-group I antiserum, which also revealed staining of phagosomes and in some areas diffuse background staining of soluble antigen. Some organisms remained unstained with the specific antiserum; these were revealed by progressive hemalum staining. In other sections, some organisms stained specifically with rabbit anti-.mu. chain serum but not with anti-.gamma. chain serum, this result suggesting that that the organisms were coated with patient''s IgM specific antibody.