Improved Purification of Rice Dwarf Virus by the Use of Polyethylene Glycol

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Abstract
Purification of rice dwarf virus (RDV) was simplified and greater yield and infectivity of virus were obtained by precipitating RDV with polyethylene glycol 6000 (PEG 6000) from clarified extracts, than by previous procedures employing differential centrifugation. The optimal conditions for use of PEG 6000 were a pH of about 7.0, 0.3 M NaCl, 6% (wt/vol) PEG 6000, and incubation at 4.degree. C for 4 h. The optimal ratio of buffer solution to tissue (vol/wt) was 5 or 6. Yields of purified virus were about 20-30% of that in the clarified extracts. Both clarified extracts and purified preparations contained (5.40-9.18) .times. 108 virions/ml at the dilution end point for infectivity. Clarified extracts and purified preparations were quick-frozen in dry-ice ethanol and stored at -72.degree. C for at least 18 mo. without detectable loss of infectivity.

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