THE REFRACTOMETRIC DETERMINATION OF ALBUMIN, GLOBULIN, AND NONPROTEIN

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Abstract
The microrefractometric method recently described by T. Brailsford Robertson1has enabled us to determine the amount of albumin and globulin in normal human serum. The specimens of serum which we have employed for this purpose have been obtained from students, nurses and doctors working in the hospital, who are in good health. No serums of patients have been used to establish these normal valuations. In brief, the method is as follows: Blood is drawn into centrifuge tubes, allowed to clot, and centrifugalized. A portion of the clear serum is treated with an equal volume of saturated ammonium sulphate to precipitate the globulins, and the precipitate removed by centrifugalizing. The clear fluid is diluted to one-half and read in the refractometer. This reading, corrected for ammonium sulphate, gives the total albumin plus the nonproteins. The nonprotein value is determined from the clear fluid remaining after the proteins have been removed

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