Capillary Electrophoresis Immunoassay for 2, 4-Dichlorophenoxyacetic Acid

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Abstract
A capillary electrophoresis (CE) immunoassay format for 2, 4-dichlorophenoxyacetic acid (2, 4-D) is demonstrated. A fluorescent labeled 2, 4-D analog competes with the analyte of interest for a finite number of binding sites provided by anti-2, 4-D monoclonal antibodies. CE then provides a means of separating and measuring both the free and antibody-bound fluorescent tracer using laser-induced fluorescence detection. For this assay format, the amount of free tracer is a sensitive indicator for the concentration of analyte present in the sample. A sequential injection format allows the rapid analysis of a small number of samples. The dynamic concentration range for 2, 4-D in either buffer or river water is 5 ppb to 1000 ppb.