Contributions of the ionization states of acidic residues to the stability of the coiled coil domain of matrilin‐1
Open Access
- 5 March 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 446 (1) , 75-80
- https://doi.org/10.1016/s0014-5793(99)00186-6
Abstract
The pK a values of eight glutamic acid residues in the homotrimeric coiled coil domain of chicken matrilin‐1 have been determined from 2D H(CA)CO NMR spectra recorded as a function of the solution pH. The pK a values span a range between 4.0 and 4.7, close to or above those for glutamic acid residues in unstructured polypeptides. These results suggest only small favorable contributions to the stability of the coiled coil from the ionization of its acidic residues.Keywords
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