Enzyme-microenvironment dynamic interactions in microstructured media

Publisher Website
Google Scholar
Abstract
A new approach for the study of an enzyme's relationship with its own reaction medium is described. The microheterogeneous (micellar and liquid crystalline) media octyl-PD-glucoside /water (or glucose in water)/octanol plus the were used to stud enzyme-microenvironment hydrolyses octyl-JD-glucoside to form glucose transition from a one-phase domain of the diagram to a polyphasic system. Starting from each homogeneous region of the phase diagram, the enzyme, due to its catalytic activity, was responsible for different phase changes. The physicochemical properties of the medium changed in proportion to the progress of the enzymatic reaction. The modifications of the microenvironment were correlated with kinetic patterns specific for each phase exploration. Enzymatic reaction rate modifications occuring during the transitions were function of the different microstructured phases in presence but they were also dependent on local concentration and disponibility of octyl-ED-glucoside monomer.

This publication has 0 references indexed in Scilit: